![]() ![]() Not more than about 0.13 m/sec per micron away from the vicinity of the exit orifice, Not more than about 0.10 m/sec per micron away from the vicinity of the exit orifice, Not more than about 0.05 m/sec per micron away from the vicinity of the exit orifice, Not more than about 0.16 m/sec per micron, A flow cytometer system as described in claim 5 wherein said nozzle has acceleration values caused by its internal surface and wherein said acceleration values are selected from a group comprising: A flow cytometer system as described in claim 4 wherein said first axial motion surface comprises a first axial acceleration surface and wherein said second axial motion surface comprises a second axial acceleration surface.Ħ. a limited maximal acceleration differentiation transition area between said first axial motion surface in said nozzle and said second axial motion surface in said nozzle wherein said limited maximal acceleration differentiation transition area is coordinated with said sample so as to be affirmatively limited to maintain a functional sample over its length.ĥ. a second axial motion surface in said nozzle andĬ. a first axial motion surface in said nozzle ī. A flow cytometer system as described in claim 1 and further comprising:Ī. A flow cytometer system as described in claim 2 wherein said single torsional interior surface element comprises a tapered single torsional interior surface element and wherein said tapered single torsional interior surface element is of an ellipse-shape or an oval-shape.Ĥ. A flow cytometer system as described in claim 1 wherein said single torsional orientation nozzle comprises a single torsional interior surface element.ģ. an analytical system which senses below said single torsional orientation nozzle.Ģ. a single torsional orientation nozzle located at least in part below said injection point andĮ. a sheath fluid port connected to said sheath fluid container ĭ. a sheath fluid container having a bottom end and wherein said sample injection tube is located within said sheath fluid container Ĭ. a sample injection tube having an injection point through which a sample may be introduced ī. Sex preseletion in rabbits: Live births from X and Y sperm seperated by DNA and cell sorting. Flow cytometry of X- and Y- chromosome bearing sperm for DNA using an improved preparation method and staining with Hoechst 333-42. Modification of a laser-based flow cytometer for high resolution DNA analysis of mammalian spermatozoa. Fluidic and optical modifications to a FACS IV for flow sorting of X- and Y- chromosome bearing sperm based on DNA. Improved flow sorting resolution of X- and Y- chromosome bering viable sperm separation using dual staining and dead cell gating. Rens, W., et al., "Improved Flow Cytometric Sorting of X-and Y-Chromosome Bearing Sperm: Substantial Increase in Yield of Sexed Semen", Molecular Reproduction and Development, 1999, pp 50-56. A., "Recent improvements in efficiency of flow cytometric sorting of X and Y- chromosome bering sperm of domestic animals: a review", 1998, New Zealand Society of Animal Protection, three pages. Rens, W., et al., "A Novel Nozzle for More Efficient Sperm Orientation to Improve Sorting Efficiency of X- and Y- Chromosome-Bearing Sperm", Technical Notes, Cytometry 33, 1998, pp 476-481. Kachel, V., et al., "Uniform Lateral Orientation, Cused by Flow Forces, of Flat Particles in Flow-Through System", The Journal of Histochemistry and Cytochemistry, 1997, vol. Enhanced flow cytometric sorting of manunalian X and Ysperm: high speed sorting and orienting no77.1e for artificial insemination. ![]() Artifical insemination of heifers with cooled, unfrozen, sexed semen. Hydrodynamic orientation of spermatozoa heads for flow cytometry. Method of treating collected mammal semen and separating sperm into X Y componentsĭean, P. Optical features of flow cytometry apparatus Particle detecting instrument with sapphire detecting cell defining a rectangular flow path Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex High speed flow cytometric separation of viable cells Sample introduction system for a flow cytometer Methods for determining antibodies specific for sex associated sperm membrane proteins Sample introduction apparatus for a flow cytometerįlow sorter with video-regulated droplet spacing ![]() Flow cytometry nozzle for high efficiency cell sorting
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